Table 1 Primer sets for the PCR amplification and sequencing of vector-borne infective agents used in the study

Babesia spp.

PIRO-A: 5'-AAT ACC CAA TCC TGA CAC AGG G-3'

PIRO-B: 5'-TTA AAT ACG AAT GCC CCC AAC-3'

[44]

Anaplasma spp./Ehrlichia spp.

EHR16SD: 5'-GGT ACC YAC AGA AGA AGT CC-3'

EHR16SR: 5'-TAG CAC TCA TCG TTT ACA GC-3'

[45, 46]

Hepatozoon spp.

HEP-F: 5'-ATA CAT GAG CAA AAT CTC AAC-3'

HEP-R: 5'-CTT ATT ATT CCA TGC TGC AG-3'

[47, 48]

Leishmania spp.

ITS-219F: 5'-AGC TGG ATC ATT TTC CGA TG-3'

ITS-219R: 5'-ATC GCG ACA CGT TAT GTG AG-3'

[49]