Bartonella vinsonii subsp. berkhoffii and Bartonella henselae bacteremia in a father and daughter with neurological disease

Table 1 Serological, culture and molecular test results for a 50-year-old veterinarian (the father) with chronic weight loss and progressive neurological dysfunction

	*Bartonella* IFA Reciprocal Titers			PCR/DNA Sequencing Results
Date/Sample (Father)	*B. henselae*	*Bvb* Genotype II	*Bvb* Genotype III	Direct Extraction	BAPGM Enrichment Culture	Subculture Isolate
10-18-07 Blood	512	32	256	Bvb TII	Neg	NIO
11-02-07 Blood	8192	64	128	Neg	Bvb TII	NIO
11-05-07 CSF	NT	NT	NT	Neg	Bvb TII	NIO
12-11-07 Oral Swab	NT	NT	NT	Bvb TII	N/A	N/A
1-18-08 Blood	1024	32	128	Neg	Bh H1	NIO
5-27-08 Blood	<16	16	16	Neg	Neg	NIO
11-04-08 Blood	<16	<16	<16	Neg	Neg	NIO

Neg = DNA was not amplified using Bartonella 16S-23S intergenic spacer (ITS) primers.
NIO = No isolate obtained by subculture following BAPGM (Bartonella alpha Proteobacteria growth medium) enrichment culture.
N/A = Not applicable
NT = Not tested
Bvb II = Bartonella vinsonii subsp. berkhoffii Genotype II by DNA sequencing
Bh H1 = Bartonella henselae ITS Houston I like strain by DNA sequencing

ISSN: 1756-3305