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Table 1 Primers and probes used for the A. platys (Apl) and Ehrlichia spp. (Ehr) PCR assays [19, 20].

From: Experimental infection and co-infection of dogs with Anaplasma platys and Ehrlichia canis: hematologic, serologic and molecular findings

Name

Sequence (5' to 3')

Apl forward primer

CCGGCGTTTAGTAAGATAAATG

Apl reverse primer

GCAAATTTAACGATCTCCGCC

Apl probe 1 FITC

ACAGTATCGGGGTAGCGAGAGTAGAA

Apl probe 2 LC670

GGAGATCGGCTATGAACAGTTCAAGAC

Ehr1 forward primer

CAGAGTGCTTCTCAGTGTAACGA

Ehr2 reverse primer

TCGCAGTTAAAATAGAACATGTAGTTG

Ehr3 forward primer

CAGAGTGCTTCTCAATGTAACGA

Ehr4 reverse primer

TTGCGGTTAAGATAGAACATGTAGTTG

Roche UPL probe #9

Catalog # 04685075001

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Parasites & Vectors

ISSN: 1756-3305

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