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Table 1 Tick cell lines used in the low temperature storage experiments: species and geographic origin, passage level, culture and storage conditions.

From: Cold storage and cryopreservation of tick cell lines

Cell line

Tick species (geographic origin)

Passage level

Culture medium

Incubation temperature

Storage conditions

BDE/CTVM 12

R. (B.) decoloratus (Kenya)

10-12

H-Lac

32°C

-196°C

BDE/CTVM 14

R. (B.) decoloratus (Kenya)

28

H-Lac

28°C

6°C

BME/CTVM 2

R. (B.) microplus (Costa Rica)

102-104

L-15

28°C

-196°C

BME/CTVM 6

R. (B.) microplus (Colombia)

174

L-15

28°C

6°C

IRE/CTVM 20

I. ricinus (United Kingdom)

118-122

L-15/L-15B1

28°C

6°C and -196°C

IDE 8

I. scapularis (North America)

103-104

L-15B

32°C

6°C and -196°C

  1. 1. Mixture of equal volumes of complete L-15 and L-15B