Comparison of wing-spot patterns (dorsal view). Typical An. takasagoensis (upper right), typical An. diru s (upper left, both adapted with permission from Sallum et al. ), An. dirus analyzed in this study (black border), and An. aff. takasagoensis (red border). Anopheles aff. takasagoensis exhibited the same spot patterns as typical An. takasagoensis: presector dark spot on vein R that does not extend or barely extends basally beyond the presector pale spot on the costa, a pale fringe spot between veins 1A and Cu2, and an accessory sector pale spot on the subcosta. Some samples from Nghệ An Province (PM01914, PM01804, PM01805, and PM01867) also exhibited spot patterns similar to those of An. takasagoensis, but they were identified as An. dirus by molecular analyses.