Role of sand lizards in the ecology of Lyme and other tick-borne diseases in the Netherlands

Tijsse-Klasen, Ellen; Fonville, Manoj; Reimerink, Johan HJ; Spitzen - van der Sluijs, Annemarieke; Sprong, Hein

doi:10.1186/1756-3305-3-42

Parasites & Vectors

Table 1 Primers and probes used in this study for PCR and RLB. Reverse primers were labeled with Biotin-tetraethyleneglycol. All probes were 5'-amino-labeled.

From: Role of sand lizards in the ecology of Lyme and other tick-borne diseases in the Netherlands

Name	Sequence (5' - 3')	Type	Target	Species	Reference
5S borSeq	GAGTTCGCGGGAGAGTAGGTTATTGCC⁽¹⁾	Primer	23S-5S IGS	B. burgdorferi sensu lato	[52]
23S borSeq	TCAGGGTACTTAGATGGTTCACTTCC	Primer	23S-5S IGS	B. burgdorferi sensu lato	[52]
A-borsl1	CTTTGACCATATTTTTATCTTCCA	Probe	23S-5S IGS	B. burgdorferi sensu lato	[68]
A-borsl2	CTTCCATCTCTATTTAGCCAATTT	Probe	23S-5S IGS	B. burgdorferi sensu lato	[52]
A-borsl3	TATTTTTATCTTCCATCTCTATTTT	Probe	23S-5S IGS	B. burgdorferi sensu lato	[52]
B31-A-s. stricto	AACACCAATATTTAAAAAACATAA	Probe	23S-5S IGS	B. burgdorferi sensu stricto	[68]
Ga2-garinii	AACATGAACATCTAAAAACATAAA	Probe	23S-5S IGS	B. garinii	[68]
Vs46lN2afzelii	AACATTTAAAAAATAAATTCAAGG	Probe	23S-5S IGS	B. afzelii	[68]
VsII62 val	CATTAAAAAAATATAAAAAATAAATTTAAGG	Probe	23S-5S IGS	B. valaisiana	[68]
A-Ruski	GAATAAAACATTCAAATAATATAAAC	Probe	23S-5S IGS	B. afzelii (variant ruski)	[69]
A-LusiP	CAAAAAAATGAACATTTAAAAAC	Probe	23S-5S IGS	B. lusitaniae	[58]
B-GA1b	CGGGATCCCGAGTTTGCCGGGACTTCTTCT⁽¹⁾	Primer	16SrRNA	Ehrlichia/Anaplasma	[57]
16S8Fe	GGAATTCAGAGTTGGATCMTGGYTCAG	Primer	16SrRNA	Eubacteria	[70]
Ehr-all	TTATCGCTATTAGATGAGCC	Probe	16SrRNA	Anaplasma genus	[57]
A-HGE	GCTATAAAGAATAGTTAGTGG	Probe	16SrRNA	HGE agent	[57]
A-Eqph	TTGCTATAAAGAATAATTAGTGG	Probe	16SrRNA	A. phagocytophilum	[57]
A-dHGE	GCTATGAAGAATAGTTAGTG	Probe	16SrRNA	HGE agent (variant)	[57]
A-dPh	TTGCTATGAAGAATAATTAGT	Probe	16SrRNA	A. phagocytophilum variant	[52]
A-E. Schot	GCTGTAGTTTACTATGGGTA	Probe	16SrRNA	E. schotti (variant)	[57]
A-murisT	AGCTATAGGTTTGCTATTAGT	Probe	16SrRNA	E. muris T variant	[69]
A-Chaff	ACCTTTTGGTTATAAATAATTGTTA	Probe	16SrRNA	E. chaffeensis	[57]
A-can	TCTGGCTATAGGAAATTGTTA	Probe	16SrRNA	E. canis	[57]
A-Wolbach	CTACCAAGGCAATGATCTA	Probe	16SrRNA	Wolbachia	[52]
Rick-16S rev	ACTCACTCGGTATTGCTGGA⁽¹⁾	Primer	16SrRNA	Rickettsia genus	[58]
Rick-16S for	AACGCTATCGGTATGCTTAACA	Primer	16SrRNA	Rickettsia genus	[58]
A-Rickall	TTTAGAAATAAAAGCTAATACCG	Probe	16SrRNA	Rickettsia genus	[58]
A-Rhelv2	GCTAATACCATATATTCTCTATG	Probe	16SrRNA	R. helvetica	[58]
A-Rconor	CTTGCTCCAGTTAGTTAGT	Probe	16SrRNA	R. conorii	[58]
A-16SRickIRS	GTATATTCTCTACGGAAAAAA	Probe	16SrRNA	Rickettsia IRS3	[58]
A-RProwaz	CGGATTAACTAGAGCTCGCT	Probe	16SrRNA	Rickettsia prowazekii	This study
A-RTyphi	CGGATTAATTAGAGCTTGCT	Probe	16SrRNA	Rickettsia typhi	This study
A-NonHelv A	AATACCGTATATTCTCTACGGA	Probe	16SrRNA	Non-Rickettsia helvetica	This study
A-NonHelv B	AATACCGTATATTCTCTGCGGA	Probe	16SrRNA	Non-Rickettsia helvetica	This study
BATH-Rn	TAAGAATTTCACCTCTGACAGTTA⁽¹⁾	Primer	18SrRNA	Babesia genus	[55]
BATH-Fn	ACACAGGGAGGTAGTGACAAG	Primer	18SrRNA	Babesia genus	[55]
Catch all 2	GTAATGGTTAATAGGARCRGTT	Probe	18SrRNA	Babesia genus	[55]
Ba-div	GTTAATATTGACTAATGTCGAG	Probe	18SrRNA	B. divergens	[71]
Ba-mic 1	CCGAACGTTATTTTATTGATTT	Probe	18SrRNA	B. microti	This study
Ba-mot	GCTTGCTTTTTTGTTACTTTG	Probe	18SrRNA	B. motasi	[55]
Ba-mic 2	GRCTTGGCATCWTCTGGA	Probe	18SrRNA	B. microti	[55]
Ba-EU1	CTGCGTTATCGAGTTATTG	Probe	18SrRNA	B. EU1	This study

⁽¹⁾ Reverse primer 5'-labeled with biotine tetraethyleneglycol

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ISSN: 1756-3305

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