Table 1 Primers used in Sarcoptes scabiei qPCR and sequencing.
From: Increased transcription of Glutathione S-transferases in acaricide exposed scabies mites
Gene | Accession number | Forward Primer (5'-3') | Reverse Primer (5'-3') | qPCR primer combination (product length) | Sequencing primer combination (product length) |
|---|---|---|---|---|---|
GST mu 1 | AF462190 | F1: GCTATTGGGATCTTCGTGGA | R1:TGCCCAAATACCGGAGAATA | F1/R1 (228 bp) | F1/R2 (628 bp) |
| Â | Â | Â | R2: TGTATTCCATTTCGCCATTG | Â | Â |
GST mu 2 | AY825933 | F1: GCCCATCAGAATGATGCTTT | R1: TTCTCAAGATAATCTGGCTTTA | F1/R1 (344 bp) | F1/R2 (519 bp) |
| Â | Â | Â | R2: GAATCGATTAACATAGTTGCC | Â | Â |
GST mu 3 | AY825934 | F1: ATCTGGCGTGCAGATAAAC | R1: CTCGAGCCTTCTCGAAATTG | F1/R1 (241 bp) | F2/R2 (929 bp) |
| Â | Â | F2: ACGCAGTTTTGTTTCGTTGG | R2: GTCTGGATTTGTTCCGTGGT | Â | Â |
GST delta 1 | AY825935 | F1: TGGACCAACATTAGCCGATA | R1: TTGCATTTGTTGAGCGAATC | F1/R1 (193 bp) | F2/R1 (631 bp) |
| Â | Â | F2: CAGAAAGTGCACCATGTCGT | Â | Â | Â |
GST delta 2 | AY825936 | F1: AGCTCAAACCGATGAGCCTA | R1:GCGAATGCAATGATGTTAGC | F1/R1 (192 bp) | F2/R1 (652 bp) |
| Â | Â | F2:TGGGTTCTATTCGACCGATAA | Â | Â | Â |
GST delta 3 | AY825937 | F1: ATGGAGGTGGTTTGAACGAG | R1: TCGTGATCGACAGCATTCAT | F1/R1 (244 bp) | F2/R1 (580 bp) |
| Â | Â | F2: AGAGAGCCCACCATGTCGTA | Â | Â | Â |
P-glycoprotein | DQ146410 | AGGCAACTTCAGCACTCGAT | ACATTCTGACCGCCATCAAT | 155 bp | Â |
SsCl | EF611372 | TGATTTCTATATGTCGGGCCATTTG | CAGGGAACCAAAGATCAACA | 329 bp | Â |
β-actin | EU624346 | CAACCATCCTTCTTGGGTATG | CCAGCTTCGTCGTATTCTTGT | 311 bp |  |