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Figure 2 | Parasites & Vectors

Figure 2

From: Characterization of the Taenia spp HDP2 sequence and development of a novel PCR-based assay for discrimination of Taenia saginata from Taenia asiatica

Figure 2

(A) Differential diagnosis of T. saginata and T. asiatica isolates from Asia using the HDP2-multiplex-PCR [22]. Lanes 1, 3, 6, 7, 9, 11, 12, 13, 15, 16, 17, 18, 19, T. asiatica; lanes 2, 4, 5, 8, 10, 14, T. saginata.; C+, T. saginata control sample (Spanish origin). Amplification products were fractionated by electrophoresis in a 1% (w/v) agarose gel and stained with ethidium bromide. The numbers on the left indicate the sizes (in bases pairs, bp) of molecular weight markers. (B) Differential diagnosis of T. saginata and T. asiatica isolates from Asia by multiplex-PCR [6]. Lanes 1, 3, 6, 7, 9, 11, 12, 13, 15, 16, 17, 18, 19, T. asiatica; lanes 2, 4, 5, 8, 10, 14, T. saginata. C1 control sample (T. solium Venezuelan origin); C2 control sample (T. solium Mexican origin); C3 control sample (T. saginata Spanish origin). Amplification products were fractionated by electrophoresis in a 2% (w/v) agarose gel and stained with ethidium bromide. The numbers on the left indicate the sizes (in bases pairs, bp) of molecular weight markers. (C) Differential diagnosis of Taenia spp. DNA samples using the HDP2 PCR III. 10 ng gDNA from Asian isolates of T. asiatica (lanes 1, 3, 6, 7, 9, 11, 12, 13, 15, 16, 17, 18 and 19); Asian isolates of T. saginata (lanes 2, 4, 5, 8, 10 and 14); T. solium (Venezuelan and Mexican origin, C1 and C2); and T. saginata (Spanish origin, C3) were amplified by HDP2-PCR. The amplification products were fractionated by electrophoresis in a 1% (w/v) agarose gel and stained using ethidium bromide. The numbers on the left indicate the sizes (in bases pairs, bp) of molecular weight markers.

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