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Table 2 PCR names, primer sequences and amplification conditions used.

From: Characterization of the Taenia spp HDP2 sequence and development of a novel PCR-based assay for discrimination of Taenia saginata from Taenia asiatica

PCR primer name Primer sequence Amplification conditions (Taq polymerase; BIOTOOLS , Spain)
HDP2 PCR I:
HDP2F2
HDP2R2
5'-GCTGTACCAGCACCTAACCRTCC-3'
5'-GCACACCGCAGCCAATTGGCTG-3'
94°C for 30 sec, 55°C for 30 sec, 72°C for 1 min, 72°C for 7 min (35 cycles)
HDP2 PCR II:
PTs7S35F1
HDP2R1
5'-CAGTGGCATAGCAG AGGAGGAA-3'
5'-GCGGAAAGTGGATCCGACTTCGATGG-3'
94°C for 30 sec, 56.5°C for 30 sec, 72°C for 2 min, 72°C for 7 min (35 cycles)
HDP2 PCR III:
HDP2F2
HDP2R3
5'-GCTGTACCAGCACCTAACCRTCC-3'
5'-ATCCTGCTCCATGTCGCTTCTAGC-3'
94°C for 30 sec, 60°C for 30 sec, 72°C for 2 min, 72°C for 7 min (35 cycles)