Table 2 PCR names, primer sequences and amplification conditions used.
PCR primer name | Primer sequence | Amplification conditions (Taq polymerase; BIOTOOLS , Spain) |
|---|---|---|
HDP2 PCR I: HDP2F2 HDP2R2 | 5'-GCTGTACCAGCACCTAACCRTCC-3' 5'-GCACACCGCAGCCAATTGGCTG-3' | 94°C for 30 sec, 55°C for 30 sec, 72°C for 1 min, 72°C for 7 min (35 cycles) |
HDP2 PCR II: PTs7S35F1 HDP2R1 | 5'-CAGTGGCATAGCAG AGGAGGAA-3' 5'-GCGGAAAGTGGATCCGACTTCGATGG-3' | 94°C for 30 sec, 56.5°C for 30 sec, 72°C for 2 min, 72°C for 7 min (35 cycles) |
HDP2 PCR III: HDP2F2 HDP2R3 | 5'-GCTGTACCAGCACCTAACCRTCC-3' 5'-ATCCTGCTCCATGTCGCTTCTAGC-3' | 94°C for 30 sec, 60°C for 30 sec, 72°C for 2 min, 72°C for 7 min (35 cycles) |