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Figure 5 | Parasites & Vectors

Figure 5

From: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region

Figure 5

PCR fragments obtained using the different AS-PCRs after separation on a 3% mixed agarose gel. (A). Detection of the L1014S mutation in An. vagus (AS-PCR I). Lane 1:100 bp ladder; Lane 2: heterozygous specimens (L1014/L1014S); Lane 3 and 4: homozygous wild type mosquitoes (L1014/L1014). (B) Detection of the L1014S mutation in An. sinensis and An. paraliae (AS-PCR II). Lane 5: heterozygous specimens (L1014/L1014S); Lane 6 and 7: homozygous wild type mosquitoes (L1014/L1014); Lane 8: 100 bp ladder. (C) Detection of the L1014S mutation in An. peditaeniatus (AS-PCR III). Lane 9: heterozygous specimens (L1014/L1014S); Lane 10 and 11: homozygous wild type mosquitoes (L1014/L1014); Lane 8: 100 bp ladder. (D) Detection of the L1014F mutation in An. peditaeniatus (AS-PCR IV). Lane 13, 15 and 16: homozygous resistant specimen (L1014F/L1014F); Lane 14: heterozygous mosquito (L1014/L1014F); Lane 17: 100 bp ladder; Lane 18: homozygous wild type mosquito (L1014/L1014).

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