Figure 5From: Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptidesBiochemical properties of recombinant BmCL1 expressed in Pichia pastoris. (A) SDS-PAGE of BmCL1 purified on ion exchange chromatography, as described in Methods. (B) Thermal inactivation at 68°C at pH 4.5. (C) Determination of the cleavage sites of SF 57-67 by BmCL1 using LC/MS.Back to article page