Figure 7From: Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptidesPeptides generated by acid hemoglobinolysis. Peptides were identified by LC-MS/MS after hemoglobinolysis with native BmAP (A), BmCL1 (B) or both enzymes (C). Digestion was performed in 100 mM citrate-phosphate buffer pH 4.5 at 37°C for up to 4 h, as described in Methods.Back to article page