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Table 1 Specific enzyme activities in the tick midgut.

From: Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptides

A       
    SF 29-35    
  pH 4.5     pH 7.0  
Enzyme activity + pepstatin + E-64   Enzyme activity + pepstatin + E-64
5,471 ± 238 320 ± 6.3a 5,295 ± 173   81 ± 25 65 ± 9.4 62 ± 7.4
    SF 57-67    
  pH 4.5     pH 7.0  
Enzyme activity + pepstatin + E-64   Enzyme activity + pepstatin + E-64
141 ± 18 139 ± 17 88 ± 14a   171 ± 21 184 ± 10 168 ± 20
B       
    SF 29-35    
  pH 4.5     pH 7.0  
Enzyme activity + pepstatin + E-64   Enzyme activity + pepstatin + E-64
6,072 ± 797 329 ± 27a 6,115 ± 206   146 ± 59 154 ± 21 109 ± 29
    SF 57-67    
  pH 4.5     pH 7.0  
Enzyme activity + pepstatin + E-64   Enzyme activity + pepstatin + E-64
138 ± 11 108 ± 6.6 33 ± 12a   319 ± 33 320 ± 20 320 ± 26
  1. Enzyme activities (RFU × 103 × min-1 × mg prot-1) were determined in midgut homogenates (A) and digest cell lysate (B) in the presence of pepstatin and E-64 at pH 4.5 and 7.0 using SF 29-35 and SF 57-67. Data represent mean ± SEM from three samples. a: p < 0.05.
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Parasites & Vectors

ISSN: 1756-3305

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