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Table 1 Specific enzyme activities in the tick midgut.

From: Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptides

A

      
   

SF 29-35

   
 

pH 4.5

   

pH 7.0

 

Enzyme activity

+ pepstatin

+ E-64

 

Enzyme activity

+ pepstatin

+ E-64

5,471 ± 238

320 ± 6.3a

5,295 ± 173

 

81 ± 25

65 ± 9.4

62 ± 7.4

   

SF 57-67

   
 

pH 4.5

   

pH 7.0

 

Enzyme activity

+ pepstatin

+ E-64

 

Enzyme activity

+ pepstatin

+ E-64

141 ± 18

139 ± 17

88 ± 14a

 

171 ± 21

184 ± 10

168 ± 20

B

      
   

SF 29-35

   
 

pH 4.5

   

pH 7.0

 

Enzyme activity

+ pepstatin

+ E-64

 

Enzyme activity

+ pepstatin

+ E-64

6,072 ± 797

329 ± 27a

6,115 ± 206

 

146 ± 59

154 ± 21

109 ± 29

   

SF 57-67

   
 

pH 4.5

   

pH 7.0

 

Enzyme activity

+ pepstatin

+ E-64

 

Enzyme activity

+ pepstatin

+ E-64

138 ± 11

108 ± 6.6

33 ± 12a

 

319 ± 33

320 ± 20

320 ± 26

  1. Enzyme activities (RFU × 103 × min-1 × mg prot-1) were determined in midgut homogenates (A) and digest cell lysate (B) in the presence of pepstatin and E-64 at pH 4.5 and 7.0 using SF 29-35 and SF 57-67. Data represent mean ± SEM from three samples. a: p < 0.05.