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Table 5 Details of selected primers and probe used to amplify Anaplasma (msp2, 16 S RNA) and Rickettsia genes (omp B,17 kDa, glt A).

From: Detection and prevalence of Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus ticks in seven study areas in Sweden

Primer Gene Nucleotide sequences (5' to 3') Product size (bp)
Apmsp2f msp2 ATG-GAA-GGT-AGT-GTT-GGT-TAT-GGT-ATT 77
Apmsp2r   TAC-GAG-CGC-TTC--AAG-ACC-AA  
Apmsp2p   TGG-TGC-CAG-GGT-TGA-GCT-TGA-GAT-TG probe
GER-3 16 S RNA TAG-ATC-CTT-CTT-AAC-GGA-AGG-GCG 151
GER-4   AAG-TGC-CCG-GCT-TAA-CCC-GCT-GGC  
EE-1 16 S RNA TCC-TGG-CTC-AGA-ACG-AAC-GCT-GGC-GGC 928
EE-2   AGT-CAC-TGA-CCC-AAC-CTT-AAA-TGG-CTG  
EE-3   GTC-GAA-CGG-ATT-ATT-CTT-TAT-AGC-TTG-C  
EE-4   CCC-TTC-CGT-TAA-GAA-GGA-TCT-AAT-CTC-C  
OMP B-IF omp B CCA-ATG-GCA-GGA-CTT-AGC-TAC-T 257
OMP B-IR   AGG-CTG-GCT-GAT-ACA-CGG-AGT-AA  
17 SFG-F 17 kDa GCT-CTTGCA-ACT-TCT-ATG-TT 431
17 SFG-R   CAT-TGT-TCG-TCA-GGT-TGG-CG  
SFG-CS-F gltA TGC-CAA-ATG-TTC-ACG-GTA-CTT-T 74
SFG-CSR   CAC-AAT-GGA-AAG-AAA-TGC-ACG-A  
SFG-CS-P   TGC-AAT-AGC-AAG-AAC-CGT-AGG-CTG-GAT-G probe