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Table 5 Details of selected primers and probe used to amplify Anaplasma (msp2, 16 S RNA) and Rickettsia genes (omp B,17 kDa, glt A).

From: Detection and prevalence of Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus ticks in seven study areas in Sweden

Primer

Gene

Nucleotide sequences (5' to 3')

Product size (bp)

Apmsp2f

msp2

ATG-GAA-GGT-AGT-GTT-GGT-TAT-GGT-ATT

77

Apmsp2r

 

TAC-GAG-CGC-TTC--AAG-ACC-AA

 

Apmsp2p

 

TGG-TGC-CAG-GGT-TGA-GCT-TGA-GAT-TG

probe

GER-3

16 S RNA

TAG-ATC-CTT-CTT-AAC-GGA-AGG-GCG

151

GER-4

 

AAG-TGC-CCG-GCT-TAA-CCC-GCT-GGC

 

EE-1

16 S RNA

TCC-TGG-CTC-AGA-ACG-AAC-GCT-GGC-GGC

928

EE-2

 

AGT-CAC-TGA-CCC-AAC-CTT-AAA-TGG-CTG

 

EE-3

 

GTC-GAA-CGG-ATT-ATT-CTT-TAT-AGC-TTG-C

 

EE-4

 

CCC-TTC-CGT-TAA-GAA-GGA-TCT-AAT-CTC-C

 

OMP B-IF

omp B

CCA-ATG-GCA-GGA-CTT-AGC-TAC-T

257

OMP B-IR

 

AGG-CTG-GCT-GAT-ACA-CGG-AGT-AA

 

17 SFG-F

17 kDa

GCT-CTTGCA-ACT-TCT-ATG-TT

431

17 SFG-R

 

CAT-TGT-TCG-TCA-GGT-TGG-CG

 

SFG-CS-F

gltA

TGC-CAA-ATG-TTC-ACG-GTA-CTT-T

74

SFG-CSR

 

CAC-AAT-GGA-AAG-AAA-TGC-ACG-A

 

SFG-CS-P

 

TGC-AAT-AGC-AAG-AAC-CGT-AGG-CTG-GAT-G

probe