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Table 5 Details of selected primers and probe used to amplify Anaplasma (msp2, 16 S RNA) and Rickettsia genes (omp B,17 kDa, glt A).
| Primer | Gene | Nucleotide sequences (5' to 3') | Product size (bp) |
|---|---|---|---|
| Apmsp2f | msp2 | ATG-GAA-GGT-AGT-GTT-GGT-TAT-GGT-ATT | 77 |
| Apmsp2r | TAC-GAG-CGC-TTC--AAG-ACC-AA | ||
| Apmsp2p | TGG-TGC-CAG-GGT-TGA-GCT-TGA-GAT-TG | probe | |
| GER-3 | 16 S RNA | TAG-ATC-CTT-CTT-AAC-GGA-AGG-GCG | 151 |
| GER-4 | AAG-TGC-CCG-GCT-TAA-CCC-GCT-GGC | ||
| EE-1 | 16 S RNA | TCC-TGG-CTC-AGA-ACG-AAC-GCT-GGC-GGC | 928 |
| EE-2 | AGT-CAC-TGA-CCC-AAC-CTT-AAA-TGG-CTG | ||
| EE-3 | GTC-GAA-CGG-ATT-ATT-CTT-TAT-AGC-TTG-C | ||
| EE-4 | CCC-TTC-CGT-TAA-GAA-GGA-TCT-AAT-CTC-C | ||
| OMP B-IF | omp B | CCA-ATG-GCA-GGA-CTT-AGC-TAC-T | 257 |
| OMP B-IR | AGG-CTG-GCT-GAT-ACA-CGG-AGT-AA | ||
| 17 SFG-F | 17 kDa | GCT-CTTGCA-ACT-TCT-ATG-TT | 431 |
| 17 SFG-R | CAT-TGT-TCG-TCA-GGT-TGG-CG | ||
| SFG-CS-F | gltA | TGC-CAA-ATG-TTC-ACG-GTA-CTT-T | 74 |
| SFG-CSR | CAC-AAT-GGA-AAG-AAA-TGC-ACG-A | ||
| SFG-CS-P | TGC-AAT-AGC-AAG-AAC-CGT-AGG-CTG-GAT-G | probe |
