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Table 4 Primers and probes used for genus-specific quantitative real time PCR, species-specific conventional PCR and generation of plasmid standards

From: Establishment of a minor groove binder-probe based quantitative real time PCR to detect Borrelia burgdorferi sensu lato and differentiation of Borrelia spielmanii by osp A-specific conventional PCR

Primer/Probe Sequence (5' to 3') Amplicon size
IGS-MGB Borrelia for TCC TAG GCA TTC ACC ATA GAC T 67 bp
IGS-MGB Borrelia rev TGG CAA AAT AGA GAT GGA AGA T  
IGS-MGB Borrelia probe 6-FAM-ATT ACT TTG ACC ATA TTT-MGBNFQ  
rpoB B. burgdorferi s.s. for* CTG TTG GTG AGC TTC TTA CT 308 bp
rpoB B. burgdorferi s.s. rev* TCT ACC ATA ATG AGT ATA ATG C  
rpoB B. afzelii for* AGA GTG CGT TCT GTT GGC 318 bp
rpoB B. afzelii rev* TCT ACC ATA ATG AGT ATA ATG T  
rpoB B. garinii for* GTG CGT TCT GTT GGG GAG 257 bp
rpoB B. garinii rev* AGT CCC CCT GGT CCA AGG  
rpoB B. valaisiana for* AGG AGA GTA CGT TCT GTT GGA 306 bp
rpoB B. valaisiana rev* ATA ATG GAC GTC TCT TAC TTC A  
ospA B. spielmanii for CAG TAG ATG TAC CTG GGG AAC TT 146 bp
ospA B. spielmanii rev GCT TTT ACG CCT TCC AGT ACA  
ITS2-MGB Ixodes for TGC GTC GTA GCC TTC 77 bp
ITS2-MGB Ixodes rev AAC GGC ATT CCC CTA C  
ITS2-MGB Ixodes probe 6-VIC-TCT AAG ACC TTC GCG-MGBNFQ  
  1. *: Primer sequences described by Lee et al. [21].