Figure 4

Extent and persistence of dsRNA- Vd GST-mu1 gene knockdown in adult V. destructor. (A) Vd GST-mu1 transcipt levels were determined by RT-PCR up to 72 h post-dsRNA treatment and gel loading normalized to actin. (B) Semi-quantitative Vd GST-mu1 RT-PCR band intensities were determined by densitometry and the relative abundance to the within-sample actin band calculated. The extent of Vd GST-mu1: actin knockdown is presented as a percentage for Vd GST-mu1-dsRNA injected mites and control mites injected with LacZ-dsRNA at each given time point. Data are means ± SEM (n = 3). Asterixes represent significant difference (P < 0.001) between treatments determined by Student's t-test.