Figure 1
From: Constraints to estimating the prevalence of trypanosome infections in East African zebu cattle
Success rate of detection of trypanosomes in the artificial dilution series of T. b. brucei. Cultured T. brucei brucei was diluted in cow blood at a concentration of 106 trypanosomes per millilitre and placed onto Whatman FTA cards. Lanes 1 to 3 are negative controls, lane 4 is a positive control lane 5 is a DNA marker, lanes 6 to 13 show the results of repeated PCR of a 10-7 dilution of the original stock (equivalent to 0.1 trypanosome per ml). Lanes 14 to 21 show the results of repeated PCR of a 10-6 dilution of the original stock (equivalent to 1 trypanosome per ml). Lanes 22 to 29 show the results of repeated PCR of a 10-5 dilution of the original stock (equivalent to 10 trypanosomes per ml). Lane 30 is a DNA marker.