Figure 4

Developmental expression analysis of S. japonicum (a) SjDicer and (b) SjAGO1. The mRNA expression levels were measured, based on three replicates, in the life stages: eggs, miracidium, cercariae, schistosomula, and adult worms, using quantitative RT-PCR. Expression levels were calibrated according to the comparative 2-^ΔΔCt method, using the constitutively expressed SjGAPDH gene as an endogenous control, and were normalized relative to the egg stage.