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Table 1 Oligonucleotides used to characterize SjDicer and SjAGO1/2

From: Analysis and characterization of the genes encoding the Dicer and Argonaute proteins of Schistosoma japonicum

Names

Sequences(5'-3')

The length of PCR productions

SjDicer

Primer 1

Forward

ACTGGACGCTGCCTACGAAAGAA

3512 bp

  

Reverse

TGGACTGATGATTGGGTTTTGGT

 
 

Primer 2

Forward

GTCACCAAAACCCAATCATCAG

884 bp

  

Reverse

GACGGTGATTATGTTGCTTGT

 
 

Primer 3

Forward

TGCTGTCGTGACTCCGGGTTATC

3406 bp

  

Reverse

TGGTGATTTTGGGATACAAGCAG

 
 

Primer 4

5'RACE outer

GGCAGCGTCCAGTAGGTCTAG

111 bp

  

5'RACE inner

GGGGGTGGAAGGAAGTGGTC

83 bp

 

Primer 5

3'RACE outer

TATTCGAATCTTTAGCCGGTGC

573 bp

  

3'RACE inner

GAGTGTGAATCTAAATGGCAAGCA

343 bp

 

Primer 6

Real time F

CGACCTCGACGAATGGTTGATG

167 bp

  

Real time R

GTTGATGCTGTTCGAGATGGTTG

 

SjAGO1

Primer 7

Forward

CCAGGACGAGGTTCAGAAGGT

516 bp

  

Reverse

CCATACTTCTCGACCACCGCC

 
 

Primer 8

Forward

GGCTCGTTCAGCTCCTGATCG

920 bp

  

Reverse

GACCTTCACCAACACCATCTC

 
 

Primer 9

5'RACE outer

CCTGAAGATCCGCAGCCGACT

326 bp

  

5'RACE inner

TTACGGTTCCAGAGACGGCGT

235 bp

 

Primer 10

Real time F

GAATTCAGGGTACGTCCAAAC

145 bp

  

Real time R

GTTGGTGCTGGATAAGAAACG

 

SjAGO2

Primer 11

Forward

GTTCATTGATTCAGCAGATCG

490 bp

  

Reverse

TCATACATGACGTCTGCTAGG

 
 

Primer 12

5'RACE outer

ACTTGGAGGTGGCCCATCTATG

228 bp

  

5'RACE inner

GACGGTCTATACGAGCTACGGG

173 bp

SjGAPDH

Primer 13

Real time F

CAGGGCTGCTTTTAACTCTGGTAA

101 bp

  

Real time R

GGGTGGAATCATATTGGAACATGT

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