Quantitative real time RT-PCR analysis of seven salivary gland genes of various spatial expression pattern groups. Mean fold-changes in salivary gland gene expression are indicated for various time points: one and four days post emergence, dPE and 6, 24, and 48 hours post blood meal, hPBM. All fold-changes are relative to the endogenous reference gene rpL32 (AAEL003396) and all time points are compared to the calibrator time point 1 dPE. Standard deviations from three biological replicates are expressed with error bars, except time point 4 dPE for AAEL006347, AAEL000726 and AAEL009670, where values were calculated from two biological replicates. ANOVA analysis and pair-wise Dunnett's tests were performed. Statistically-significant differences having a P value of ≤0.05 are indicated (asterisk).