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Table 1 Summary of molecular approaches used for the detection and/or genetic differentiation of Fasciola spp..

From: Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches

Molecular
approach
Species investigated Developmental stage DNA target regions References
  F. hepatica F. gigantica Intermediate
form
Adult Cercaria Eggs   
Conventional PCR      ITS2 38
    ITS2 14, 27
     ITS1, ITS2 25
      nad 1, cox 1 28
    nad 1, cox 1 13, 17
    ITS2, cox 1 9
    ITS1, nad 1 16
    cox 1, ITS1, ITS2 15
    nad 1, cox 1, ITS2, 28S 18
      Complete mitochondrial genome 30
      124 bp repetitive DNA sequence 49
      124 bp repetitive DNA sequence 50
      28S 39
Multiplex PCR      cox 1, ITS1, ITS2 52
Specific PCR ITS2 47
PCR-RFLP    ITS2 22
      cox 1, ITS2 23
    ITS1 8, 21
PCR-SSCP     Repetitive DNA sequences 46
RAPD-PCR      Random nucleotide sequence 43
    Random nucleotide sequence 53
SRAP      Random nucleotide sequence 42
    Random nucleotide sequence 10
DNA probe      Repetitive DNA fragments 48
TaqMan real-time PCR    ITS2 54
LAMP IGS 58
PCR      Microsatellites 59