Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches

Parasites & Vectors

Table 1 Summary of molecular approaches used for the detection and/or genetic differentiation of Fasciola spp..

Molecular approach	Species investigated			Developmental stage			DNA target regions	References
	*F. hepatica*	*F. gigantica*	Intermediate form	Adult	Cercaria	Eggs
Conventional PCR	√			√			ITS2	38
	√	√	√	√			ITS2	14, 27
	√	√		√			ITS1, ITS2	25
	√			√			nad 1, cox 1	28
	√	√	√	√			nad 1, cox 1	13, 17
	√	√	√	√			ITS2, cox 1	9
	√	√	√	√			ITS1, nad 1	16
	√	√	√	√			cox 1, ITS1, ITS2	15
	√	√	√	√			nad 1, cox 1, ITS2, 28S	18
	√			√			Complete mitochondrial genome	30
		√			√		124 bp repetitive DNA sequence	49
	√				√		124 bp repetitive DNA sequence	50
	√			√			28S	39
Multiplex PCR	√				√		cox 1, ITS1, ITS2	52
Specific PCR	√	√	√	√	√	√	ITS2	47
PCR-RFLP	√	√	√	√			ITS2	22
		√		√			cox 1, ITS2	23
	√	√	√	√			ITS1	8, 21
PCR-SSCP	√	√		√			Repetitive DNA sequences	46
RAPD-PCR		√		√			Random nucleotide sequence	43
	√	√	√	√			Random nucleotide sequence	53
SRAP	√			√			Random nucleotide sequence	42
	√	√	√	√			Random nucleotide sequence	10
DNA probe		√			√		Repetitive DNA fragments	48
TaqMan real-time PCR	√	√	√	√			ITS2	54
LAMP	√	√	√	√	√	√	IGS	58
PCR	√			√			Microsatellites	59

ISSN: 1756-3305