Figure 3
Detection limit of Plasmodium vivax mtDNA LAMP and nested PCR. (A) Agarose gel electrophoresis of LAMP product before (1) and after (2) digestion by Dde I (Fermentas). M: DNA ladder (1 kb Plus, Fermentas). (B) pGEM-PvMito plasmid was 10-fold serially diluted and tested by LAMP, M: DNA ladder, 1: 1.0 × 106, 2: 1.0 × 105, 3: 1.0 × 104, 4: 1.0 × 103, 5: 1.0 × 102, 6: 1.0 × 101, 7: DDW, results were observed by both electrophoresis and under blue light. (C) A P. vivax DBS sample with parasitaemia at 11,000 parasites/μl blood was serially diluted with DDW and tested by LAMP and nested PCR: M: DNA ladder, 1, undiluted, 2: 1:10, 3, 1:100, 4, 1:1,000, 5, 1:10,000; 6, DDW. (D) The same P. vivax DBS sample of (C) was diluted using mixed negative DBS sample and was tested as (C).