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Table 1 PCR conditions and primers used for the detection of Babesia spp., Rickettsia spp. and Bartonella spp.

From: Occurrence of Babesia spp., Rickettsia spp. and Bartonella spp. in Ixodes ricinus in Bavarian public parks, Germany

pathogen primer gene (bp) ref. final primer
conc.
Ds As Es cycles
Babesia spp. BJ1 5'-GTCTTGTAATTGGAATGATGG-3'
BN2 5'-TAGTTTATGGTTAGGACTACG-3'
18S rRNA
(411-452)
[12] 1 μM 94°C 30 s 55°C 30 s 72°C 40 s 40
Rickettsia spp. RpCS.877p 5'-GGGGGCCTGCTCACGGCGG-3'
RpCS.1258n 5'-ATTGCAAAAAGTACAGTGAACA-3'
gltA (380) [54] 0,5 μM 95°C 20 s 48°C 30 s 60°C 2 min 35
  120-2788 5'-AAACAATAATCAAGGTACTGT-3'
120-3599 5'-TACTTCCGGTTACAGCAAAGT-3'
ompB (765) [55] 0,5 μM 95°C 30 s 50°C 30 s 68°C 90 s 40
Bartonella spp. BA325s 5'-CTTCAGATGATGATCCCAAGCCTTCTGGCG-3'
BA1100as 5'-GAACCGACGACCCCCTGCTTGCAAAGCA-'3
16S-23S rRNA (ITS) (420-780) [53] 1 μM 94°C 30 s 66°C 30 s 72°C 50 s 40
  1. Ds: denaturation step; As: annealing step; Es: elongation step