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Table 1 PCR conditions and primers used for the detection of Babesia spp., Rickettsia spp. and Bartonella spp.

From: Occurrence of Babesia spp., Rickettsia spp. and Bartonella spp. in Ixodes ricinus in Bavarian public parks, Germany

pathogen

primer

gene (bp)

ref.

final primer

conc.

Ds

As

Es

cycles

Babesia spp.

BJ1 5'-GTCTTGTAATTGGAATGATGG-3'

BN2 5'-TAGTTTATGGTTAGGACTACG-3'

18S rRNA

(411-452)

[12]

1 μM

94°C 30 s

55°C 30 s

72°C 40 s

40

Rickettsia spp.

RpCS.877p 5'-GGGGGCCTGCTCACGGCGG-3'

RpCS.1258n 5'-ATTGCAAAAAGTACAGTGAACA-3'

gltA (380)

[54]

0,5 μM

95°C 20 s

48°C 30 s

60°C 2 min

35

 

120-2788 5'-AAACAATAATCAAGGTACTGT-3'

120-3599 5'-TACTTCCGGTTACAGCAAAGT-3'

ompB (765)

[55]

0,5 μM

95°C 30 s

50°C 30 s

68°C 90 s

40

Bartonella spp.

BA325s 5'-CTTCAGATGATGATCCCAAGCCTTCTGGCG-3'

BA1100as 5'-GAACCGACGACCCCCTGCTTGCAAAGCA-'3

16S-23S rRNA (ITS) (420-780)

[53]

1 μM

94°C 30 s

66°C 30 s

72°C 50 s

40

  1. Ds: denaturation step; As: annealing step; Es: elongation step