pathogen | primer | gene (bp) | ref. | final primer conc. | Ds | As | Es | cycles |
---|---|---|---|---|---|---|---|---|
Babesia spp. | BJ1 5'-GTCTTGTAATTGGAATGATGG-3' BN2 5'-TAGTTTATGGTTAGGACTACG-3' | 18S rRNA (411-452) | [12] | 1 μM | 94°C 30 s | 55°C 30 s | 72°C 40 s | 40 |
Rickettsia spp. | RpCS.877p 5'-GGGGGCCTGCTCACGGCGG-3' RpCS.1258n 5'-ATTGCAAAAAGTACAGTGAACA-3' | gltA (380) | [54] | 0,5 μM | 95°C 20 s | 48°C 30 s | 60°C 2 min | 35 |
 | 120-2788 5'-AAACAATAATCAAGGTACTGT-3' 120-3599 5'-TACTTCCGGTTACAGCAAAGT-3' | ompB (765) | [55] | 0,5 μM | 95°C 30 s | 50°C 30 s | 68°C 90 s | 40 |
Bartonella spp. | BA325s 5'-CTTCAGATGATGATCCCAAGCCTTCTGGCG-3' BA1100as 5'-GAACCGACGACCCCCTGCTTGCAAAGCA-'3 | 16S-23S rRNA (ITS) (420-780) | [53] | 1 μM | 94°C 30 s | 66°C 30 s | 72°C 50 s | 40 |