Table 1 Primer sets for the PCR amplification and sequencing of canine TBD pathogens used in this study.

Babesia species

BTF1: 5'-GGC TCA TTA CAA CAG TTA TAG-3'

[34]

BTR1: 5'-CCC AAA GAC TTT GAT TTC TCT C-3'

BTF2: 5'-CCG TGC TAA TTG TAG GGC TAA TAC-3'

BTR2: 5'-GGA CTA CGA CGG TAT CTG ATC G-3'

Ehrlichia canis

ECA: 5'-AAC ACA TGC AAG TCG AAC GGA-3'

[36]

HE3: 5'-TAT AGG TAC CGT CAT TAT CTT CCC TAT-3'

Hepatozoon species

HEP-F: 5'-ATA CAT GAG CAA AAT CTC AAC-3'

[38]

HEP-R: 5'-CTT ATT ATT CCA TGC TGC AG-3'

Anaplasma platys

Platys: 5'-GAT TTT TGT CGT AGC TTG CTA TG-3'

[37]

EHR16SR: 5'TAG CAC TCA TCG TTT ACA GC-3'

Mycoplasma species

HBT-F: 5'-ATA CGG CCC ATA TTC CTA CG-3'

[39]

HBT-R: 5'-TGC TCC ACC ACT TGT TCA-3'