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Figure 2 | Parasites & Vectors

Figure 2

From: Molecular characterization of cathepsin B from Clonorchis sinensis excretory/secretory products and assessment of its potential for serodiagnosis of clonorchiasis

Figure 2

Expression, purification and identification of Cs CB. A. Expression and purification of Cs CB by 12% SDS-PAGE. M, protein molecular weight markers; lane 1, lysate of E. coli with pET-28a(+) without IPTG induction; lane 2, lysate of E. coli with pET-28a(+) with IPTG induction, lane 3, lysate of E. coli with pET28a(+)-Cs CB before induction; lane 4, lysate of E. coli with pET28a(+)-Cs CB after induction; lane 5, supernatant of induced pET28a(+)-Cs CB; lane 6, sediment of induced pET28a(+)-Cs CB; lane 7, purified Cs CB. B. Western blotting analysis of recombinant Cs CB. Lane 1, recombinant Cs CB reacted with the sera from normal rats; lane 2, recombinant CsCB reacted with the sera from rats immunized with recombinant Cs CB; lane 3, recombinant Cs CB reacted with the sera from C. sinensis- infected rats; lane 4, recombinant Cs CB reacted with the sera from rats immunized with the ESP.

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