Transcriptional level of C. sinensis CB at metacercaria and adult worm. A. 1% agarose gel. DNA marker (M), Cs CB PCR products amplified from adult worm cDNA (lane 1), metacercaria cDNA (lane 2), β-actin amplified from adult worm cDNA (lane 3), metacercaria cDNA (lane 4) and Cs CB PCR product amplified from recombinant CB plasmid (lane 5). B. PCR products were quantified and analyzed. PCR products were quantified by Tanon Gis software, compared normalized by C. sinensis β-actin, analyzed by Student's t test (Software package SPSS16.0). P-value of < 0.05 indicated statistical significance.