Small risk of developing symptomatic tick-borne diseases following a tick bite in the Netherlands

Tijsse-Klasen, Ellen; Jacobs, Jac J; Swart, Arno; Fonville, Manoj; Reimerink, Johan H; Brandenburg, Afke H; van der Giessen, Joke WB; Hofhuis, Agnetha; Sprong, Hein

doi:10.1186/1756-3305-4-17

Parasites & Vectors

Table 1 Primers and probes used in this study for PCR and RLB.

From: Small risk of developing symptomatic tick-borne diseases following a tick bite in the Netherlands

Name	Sequence (5' - 3')	Type	Target	Species	Reference
5S borSeq	GAGTTCGCGGGAGAGTAGGTTATTGCC ⁽¹⁾	Primer	23S-5S IGS	B. burgdorferi sensu lato	[38]
23S borSeq	TCAGGGTACTTAGATGGTTCACTTCC	Primer	23S-5S IGS	B. burgdorferi sensu lato	[38]
A-borsl1	CTTTGACCATATTTTTATCTTCCA	Probe	23S-5S IGS	B. burgdorferi sensu lato	[39]
A-borsl2	CTTCCATCTCTATTTAGCCAATTT	Probe	23S-5S IGS	B. burgdorferi sensu lato	[38]
A-borsl3	TATTTTTATCTTCCATCTCTATTTT	Probe	23S-5S IGS	B. burgdorferi sensu lato	[38]
B31-A-s.stricto	AACACCAATATTTAAAAAACATAA	Probe	23S-5S IGS	B. burgdorferi sensu stricto	[39]
Ga2-garinii	AACATGAACATCTAAAAACATAAA	Probe	23S-5S IGS	B. garinii	[39]
Vs46lN2afzelii	AACATTTAAAAAATAAATTCAAGG	Probe	23S-5S IGS	B. afzelii	[39]
VsII62 val	CATTAAAAAAATATAAAAAATAAATTTAAGG	Probe	23S-5S IGS	B. valaisiana	[39]
A-Ruski	GAATAAAACATTCAAATAATATAAAC	Probe	23S-5S IGS	B. afzelii (variant ruski)	[40]
A-LusiP	CAAAAAAATGAACATTTAAAAAC	Probe	23S-5S IGS	B. lusitaniae	[41]
B-GA1b	CGGGATCCCGAGTTTGCCGGGACTTCTTCT ⁽¹⁾	Primer	16SrRNA	Ehrlichia/Anaplasma	[42]
16S8Fe	GGAATTCAGAGTTGGATCMTGGYTCAG	Primer	16SrRNA	Eubacteria	[43]
Ehr-all	TTATCGCTATTAGATGAGCC	Probe	16SrRNA	Anaplasma genus	[42]
A-HGE	GCTATAAAGAATAGTTAGTGG	Probe	16SrRNA	HGE agent	[42]
A-Eqph	TTGCTATAAAGAATAATTAGTGG	Probe	16SrRNA	A. phagocytophilum	[42]
A-dHGE	GCTATGAAGAATAGTTAGTG	Probe	16SrRNA	HGE agent (variant)	[42]
A-dPh	TTGCTATGAAGAATAATTAGT	Probe	16SrRNA	A. phagocytophilum variant	[38]
A-E.Schot	GCTGTAGTTTACTATGGGTA	Probe	16SrRNA	A. schotti (variant)	[42]
A-murisT	AGCTATAGGTTTGCTATTAGT	Probe	16SrRNA	E. muris T variant	[40]
A-Chaff	ACCTTTTGGTTATAAATAATTGTTA	Probe	16SrRNA	E. chaffeensis	[42]
A-can	TCTGGCTATAGGAAATTGTTA	Probe	16SrRNA	E. canis	[42]
A-Wolbach	CTACCAAGGCAATGATCTA	Probe	16SrRNA	Wolbachia	[38]
Rick-16S rev	ACTCACTCGGTATTGCTGGA ⁽¹⁾	Primer	16SrRNA	Rickettsia genus	[41]
Rick-16S for	AACGCTATCGGTATGCTTAACA	Primer	16SrRNA	Rickettsia genus	[41]
A-Rickall	TTTAGAAATAAAAGCTAATACCG	Probe	16SrRNA	Rickettsia genus	[41]
A-Rhelv2	GCTAATACCATATATTCTCTATG	Probe	16SrRNA	R. helvetica	[41]
A-Rconor	CTTGCTCCAGTTAGTTAGT	Probe	16SrRNA	R. conorii	[41]
A-16SRickIRS	GTATATTCTCTACGGAAAAAA	Probe	16SrRNA	Rickettsia IRS3	[41]
A-RProwaz	CGGATTAACTAGAGCTCGCT	Probe	16SrRNA	Rickettsia prowazekii	[34]
A-RTyphi	CGGATTAATTAGAGCTTGCT	Probe	16SrRNA	Rickettsia typhi	[34]
A-NonHelv A	AATACCGTATATTCTCTACGGA	Probe	16SrRNA	Non- Rickettsia helvetica	[34]
A-NonHelv B	AATACCGTATATTCTCTGCGGA	Probe	16SrRNA	Non- Rickettsia helvetica	[34]
BATH-Rn	TAAGAATTTCACCTCTGACAGTTA ⁽¹⁾	Primer	18SrRNA	Babesia genus	[44]
BATH-Fn	ACACAGGGAGGTAGTGACAAG	Primer	18SrRNA	Babesia genus	[44]
Catch all 2	GTAATGGTTAATAGGARCRGTT	Probe	18SrRNA	Babesia genus	[44]
Ba-div	GTTAATATTGACTAATGTCGAG	Probe	18SrRNA	B. divergens	[45]
Ba-mic 1	CCGAACGTTATTTTATTGATTT	Probe	18SrRNA	B. microti	[34]
Ba-mot	GCTTGCTTTTTTGTTACTTTG	Probe	18SrRNA	B. motasi	[44]
Ba-mic 2	GRCTTGGCATCWTCTGGA	Probe	18SrRNA	B. microti	[44]
Ba-EU1	CTGCGTTATCGAGTTATTG	Probe	18SrRNA	B. EU1	[34]

Probes were 5'-amino-labeled. ⁽¹⁾ Reverse primer 5'-labeled with biotine tetraethyleneglycol

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ISSN: 1756-3305

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