Immunolocalization of the TSP proteins. Immunofluorescence of adult female worms (left) and male worms (right) were performed by anti-Sj-TSP-#2, anti-Sj-TSP-#5 and anti-Sj-TSP-#6 immune sera as well as pre-immune sera followed by FITC-conjugated antibody to mouse IgG. Optical images (upper panels), corresponding fluorescence microscopy images (green, middle panels) and tissue nuclei (DAPI labeled, blue, bottom panels) of adult worms are shown. Pre-immune serum was used as negative control. A magnifications bar in panels is 100 μm.