Figure 2

Distance tree constructed for phylogenetic comparison of isolates obtained in this study. The tree generated using the Neighbor-joining algorithm and maximum likelihood method shows high phylogenetic similarity between the five viral hemorrhagic septicemia virus (VHSV) isolates obtained in this study (ON41, ON55-M, HU54-M, MI18-M, and MI27-M) and other isolates belonging to the VHSV genotype IVb. The alignment file used to produce the tree contained partial VHSV nucleoprotein (N) gene sequences (737 nucleotide positions). Snakehead rhabdovirus was used as the outgroup. The scale bar indicates the number of substitutions per nucleotide site.