FISH of microsatellites performed on the An. nili chromosomes. Hybridizations of microsatellite markers 1G13 (A), 2Ateta and 1F43 (B), and B115 (C) with polytene chromosomes are shown. Chromosomes were counterstained with the fluorophore YOYO-1 and hybridized with fluorescently labeled probes Cy5 (blue) and Cy3 (red). The top panel shows fluorescent images of chromosomes after FISH. The bottom panel shows inverted grayscale chromosome images with color labels and distinct banding patterns.