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Table 5 Information on AFLP

From: Stirred, not shaken: genetic structure of the intermediate snail host Oncomelania hupensis robertsoni in an historically endemic schistosomiasis area

Primer name

Primer sequence

Adapter

 

EcoR I Forward

5'-CTC GTA GAC TGC GTA CC-3'

EcoR I Reverse

5'-AAT TGG TAC GCA GTC TAC-3'

Mse I Forward

5'-GAC GAT GAG TCC TGA G-3'

Mse I Reverse

5'-TAC TCA GGA CTC AT-3'

Pre-selective primers

 

EcoR I-A

5'-GAC TGC GTA CCA ATT CA-3'

Mse I-C

5'-GAT GAG TCC TGA GTA AC-3'

Mse I-AC

5'-GAT GAG TCC TGA GTA AA C-3'

Selective primers

 

IRD-800 EcoR I-AAC

5'-GAC TGC GTA CCA ATT CAA C-3'

IRD-700 EcoR I-AGC

5'-GAC TGC GTA CCA ATT CAG C-3'

Mse I-CAC

5'-GAT GAG TCC TGA GTA ACA C-3'

Mse I-CAT

5'-GAT GAG TCC TGA GTA ACA T-3'

Mse I-CGT

5'-GAT GAG TCC TGA GTA ACG T-3'

MseI-ACTA

5'-GAT GAG TCC TGA GTA AAC TA-3'

MseI-ACAT

5'-GAT GAG TCC TGA GTA AAC AT-3'

  1. Adapters and PCR primer sequences used for AFLP genotyping of Oncomelania h. robertsoni.
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Parasites & Vectors

ISSN: 1756-3305

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