Figure 7

Histopathology of brain, liver and spleen of mice (40×). BALB/c mice (n = 3/group) were immunized on days 0, 14 and 28 with 100 μg pcDNA3.1-HisGRA6. Two groups of mice, Kunming Mice immunized with pcDNA3.1-HisGRA6 and BALB/c immunized with empty pcDNA3.1, were included as controls. The mice were challenged intraperitoneally with 1, 000 of T. gondii RH strain tachyzoites. One week after the challenge, different organs (brain, liver and spleen) were collected and processed for histology. Parasites are indicated by an arrow. In this figure, A1-A4: brain; B1-B4: liver; C1-C4: spleen; A1, B1, C1: BALB/c mice immunized with pcDNA(3.1)-HisGRA6 plus LMS; A2, B2, C2: BALB/c mice immunized with pcDNA(3.1)-HisGRA6; A3, B3, C3: BALB/c mice immunized with empty pcDNA3.1; A4, B4, C4: Kunming Mice immunized with pcDNA(3.1)-HisGRA6.