Skip to main content

Table 1 Primer sequences for the cytochrome b-based polymerase chain reaction blood meal identification assay

From: The abundance and host-seeking behavior of culicine species (Diptera: Culicidae) and Anopheles sinensis in Yongcheng city, people's Republic of China

1Primers

5'-3'sequences

Product size with

Unrev1025

Human741F

GGC TTA CTT CTC TTC ATT CTC TCC T

334

Cow121F

CAT CGG CAC AAA TTT AGT CG

561

Pig573F

CCT CGC AGC CGT ACA TCT C

453

Goat894F

CCT AAT CTT AGT ACT TGT ACC CTT CCT C

132

Dog368F

GGA ATT GTA CTA TTA TTC GCA ACC AT

680

Unrev1025

GGT TGT CCT CCA ATT CAT GTT A

-

  1. 1Primers of this multiplex PCR assay were designed based on the sequence differences in cytochrome b sequences.