Genetic diversity of transmission-blocking vaccine candidates Pvs25 and Pvs28 in Plasmodium vivax isolates from Yunnan Province, China

Table 2 Amino acid substitutions of Pvs28 from Yunnan P. vivax isolates

Haplotypes (number)	SS	EGF-1	EGF-2	EGF-2	EGF-3	EGF-3	RR	RR	THR
	14	52	98	105	116	140	179	#	222
Sal-I	V	M	L	E	L	T	G	6	I
Pvs28-I (n = 19)	•	L	•	•	•	S	•	5/6	•
Pvs28-II (n = 4)	•	•	I	•	V	•	•	6	•
Pvs28-III (n = 2)	L	•	•	K	V	•	•	6	•
Pvs28-IV (n = 2)	•	L	•	•	•	•	•	6	•
Pvs28-V (n = 1)	•	•	•	•	•	•	•	6	M
Pvs28-VI (n = 1)	•	•	•	•	V	•	•	6	M
Pvs28-VII (n = 1)	•	L	•	•	•	•	E	6	•

SS, secretary signal sequence; EGF, epidermal growth factor-like domain; RR, repeat regions, # indicates the number of the GSGGE/D repeats; THR, the C terminal hydrophobic region.
• indicates identical amino acid residues compared to the Salvador I strain.