Figure 4
Effect of suramin on the rate of S. cervi PFK-catalyzed reaction (A) and the Hill plot of the data presented in (A). Figure 4A. Effect of suramin on the rate of S. cervi PFK-catalyzed reaction. Suramin concentrations were nil (○), 1 (Δ) and 5 nM (●). Mg2+ concentration was constant (3.3 mM). The concentration of ATP was 0.1 mM. Enzyme concentration used was 10 μg/ml. Other conditions were same as described in Materials and Methods. Figure 4B. Hill plot of the data on the effect of suramin (0-10 nM) on the rate of S. cervi PFK-catalyzed reaction. The concentrations of F-6-P, ATP and Mg2+ were 3.3, 0.1 and 3.3 mM. Other conditions were same as described in Materials and Methods. Vi and V0 are rates of reaction in the presence and absence of suramin.