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Table 5 Effect of cAMP, AMP and ADP on the activity of S.cervi PFK under different conditions

From: Modulation of phosphofructokinase (PFK) from Setaria cervi, a bovine filarial parasite, by different effectors and its interaction with some antifilarials

Effector Concentration

(mM)

Experimental

Conditions

% of control

cAMP (0.064)

1

348

AMP (0.38)

1

181

ADP (2.50)

1

130

cAMP (0.064)

2

282

AMP (0.48)

2

50

ADP (0.50)

2

122

  1. Experimental condition 1: Enzyme activity was assayed against inhibitory concentrations of ATP (1.0 mM) and low concentration of F-6-P (0.5 mM). MgCl2 concentration was 3.3 mM in all the experiments. Experimental condition 2: Enzyme activity was assayed by the standard procedure using excess F-6-P (3.3 mM), MgCl2 (3.3 mM) and optimum level of ATP (0.1 mM).
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Parasites & Vectors

ISSN: 1756-3305

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