Skip to main content

Table 6 Effect of thiols and p-chloromercuribenzoate (p-CMB) on the activity of PFK from S.cervi

From: Modulation of phosphofructokinase (PFK) from Setaria cervi, a bovine filarial parasite, by different effectors and its interaction with some antifilarials

Compounds

Concentration (mM)

Activity remaining (%)

Control

-

100

β -ME

5

100

 

10

170

Cysteine

5

107

 

10

170

p-CMB

0.1

25

 

5.0

0.2

*p-CMB+β-ME

0.1+5.0

70

#β-ME + p-CMB

5.0+0.1

100

  1. The enzyme was pre-incubated with the effectors at 37°C for 15 min and then the activity was determined in the usual way as mentioned in Materials and Methods. *After 15 min of incubation of the enzyme with p-MB, β-ME was added, incubated for another 15 min and the reaction activity was monitored. # After 15 min incubation of the enzyme with β-ME, p-CMB was added, incubated for further 15 min and then the residual activity was monitored. Control refers to the activity in absence of any effectors.