Compounds | Concentration (mM) | Activity remaining (%) |
---|
Control | - | 100 |
β -ME | 5 | 100 |
 | 10 | 170 |
Cysteine | 5 | 107 |
 | 10 | 170 |
p-CMB | 0.1 | 25 |
 | 5.0 | 0.2 |
*p-CMB+β-ME | 0.1+5.0 | 70 |
#β-ME + p-CMB | 5.0+0.1 | 100 |
- The enzyme was pre-incubated with the effectors at 37°C for 15 min and then the activity was determined in the usual way as mentioned in Materials and Methods. *After 15 min of incubation of the enzyme with p-MB, β-ME was added, incubated for another 15 min and the reaction activity was monitored. # After 15 min incubation of the enzyme with β-ME, p-CMB was added, incubated for further 15 min and then the residual activity was monitored. Control refers to the activity in absence of any effectors.