Table 1 Primers used for amplification and sequencing of Ixodiphagus hookeri (Howard) DNA
From: Parasites of vectors - Ixodiphagus hookeri and its Wolbachia symbionts in ticks in the Netherlands
Primer | Target | sequence 5' → 3' | original name | Reference |
|---|---|---|---|---|
16S-F1 | 16S rRNA | cacctgtttatcaaaaacat | 16SWb | [32] |
16S-F2 | 16S rRNA | ctgcagtattttgactgtacaaaggtagcataatc | -- | This study |
16S-R1 | 16S rRNA | cttaattcaacatcgaggtcgc | -- | modified from [33] |
28S-F1 | 28S rRNA | aagagagagttcaagagtacgtg | -- | [34] |
28S-F2 | 28S rRNA | actttcaggacccgtcttga | -- | R/C of 28S-R1 |
28S-R1 | 28S rRNA | tcaagacgggtcctgaaagt | D2-4057 R | [35] |
28S-R2 | 28S rRNA | tagttcaccatctttcgggtccc | 28S-PM | [34] |
28S-R3 | 28S rRNA | tcggaaggaaccagctacta | D3-4413 R | [35] |