Figure 2

Taenia solium oncospheres obtained from a tapeworm carrier are seen under contrast phase microscopy, PI, FITC and a merged image of both stains. The first row corresponds to oncospheres incubated with anti GST IgG as a negative control; rows 2 and 3 include images obtained with the specific anti TSOL18 and anti TSOL45-1A antibodies, respectively. The arrows in the first column point to the hooks of the oncospheres that was used for focus. Positive reactions were detected with anti-TSOL18 and anti-TSOL45-1A antibodies only on the surface of the oncospheres, as seen in the merged image. The high intensity emission of PI in FITC sections caused non-specific background fluorescence on the same internal structures seen in PI sections.