Figure 2

Comparison of An. gambiae salivary gland profiles at the protein and antigenic level according to different preservation conditions. (A) Comparative An. gambiae salivary gland (SG) protein profiles between different preservation conditions. Salivary gland proteins were separated on 12% SDS-PAGE gels. Each protein profile corresponds to a distinct preservation condition. Lane 1: SG dissected on ice and stored at -20°C in PBS (reference); lane 2: SG dissected at RT and stored 5 days at 4°C in PBS containing protease inhibitor cocktail (PBSpi); lane 3: SG dissected at RT and stored 5 days at RT in PBSpi; lane 4: SG dissected at RT and stored 5 days at 4°C in cell lysis buffer; lane 5: SG dissected at RT and stored 5 days at RT in cell lysis buffer. Standard molecular weights (MW) are indicated at the left side in kilodaltons (kDa). (B) Schematic representations of densitometric protein profiles from the 5 salivary gland preservation conditions. The line color corresponds to the colored box used at the top of each protein profile. The arrow head indicates the band that was used for abundance comparison. A.U.: Arbitrary Unit. R.f.: Relative front of migration. (C) IgG immune profiles against An. gambiae salivary gland proteins using the pooled sera from exposed individuals. The immunoblots were performed by transferring the SDS-PAGE gel shown in (A) onto a nitrocellulose membrane. Antigenic bands detected only in samples preserved in cell lysis buffer are indicated with an asterisk (*).