Figure 1

Identification of putative FReD proteins in D. marginatus plasma and detection of HLGP in tick tissues. Molecular weights of standard proteins are depicted. A) Immunoblotting of non-reduced electrophoretically separated D. marginatus plasma proteins. 1 - D. marginatus plasma proteins, stained with Coomassie Brilliant Blue, 2 - immunostaining of plasma proteins using antibodies against HA of D. marginatus hemolymph serum, 3 - immunostaining of plasma proteins by anti-Dorin M antibodies. B) Immunoblotting of HLGP in D. marginatus tissues using mouse polyclonal anti-HLGP serum. 1 - fed female plasma, 2 - unfed female plasma, 3 - fed male plasma, 4 - unfed male plasma, 5 - fed female hemocytes, 6 - unfed female hemocytes, 7 - fed male hemocytes, 8 - unfed male hemocytes, 9 - fed female gut, 10 - fed female salivary glands. * marks the position of native glycosylated HLGP, # marks the deglycosylated form of HLGP.