Figure 3

HLGP is a glycosylated protein. Molecular weights of standard proteins are depicted. A) Schiff staining and lectin staining of electrophoretically separated and electroblotted purified HLGP. 1 - tick plasma proteins, stained with Coomassie Brilliant Blue, 2-5 electroblotted HLGP staining. 2 - Schiff staining, 3 - SNA lectin staining, 4 - GNA lectin staining, 5 - DSA lectin staining of purified HLGP. Positive reaction in Schiff staining confirms the presence of glycans on HLGP. Types of glycans identified by positive reactions in lectinoblotting are described in text. B) Immunodetection of deglycosylated purified HLGP using anti-HLGP serum. 1,3,4 - non-reduced electroeluted HLGP and 2,5,6 - reduced electroeluted HLGP stained with anti-HLGP serum. Samples in lanes 3 and 5 were deglycosylated using Endo H, samples in lanes 4 and 6 deglycosylated using PNGase A/F. * - native glycosylated HLGP, # - deglycosylated form of HLGP.