Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands

Table 1 Geographic distribution of questing I. ricinus ticks (nymphs and adults)

Location	(Previous study)	Ticks (n)	*Bartonella-* related *spp. (EF662054)*
Apeldoorn	Gassner et al., 2010 [74]	40	4
Bergherbos	Tijsse-Klasen et al., 2010 [38]	17	0
Duin en Kruidberg	Sprong et al., 2009 [37]	157	36
Ede	Gassner et al., 2010 [74]	271	14
Eijsden	Gassner et al., 2010 [74]	49	2
Gieten	Gassner et al., 2010 [74]	22	1
Haaksbergen	Gassner et al., 2010 [74]	15	0
Heumensoord	Tijsse-Klasen et al., 2010 [38]	67	5
Hoog Baarlo	Gassner et al., 2010[74]	221	12
Hullenberg	Tijsse-Klasen et al., 2010 [38]	60	6
Kwade Hoek	Gassner et al., 2010 [74]	122	15
Leusderheide	Tijsse-Klasen et al., 2010 [38]	140	12
Twiske	Gassner et al., 2010 [74]	167	25
Veldhoven	Gassner et al., 2010 [74]	16	1
Wassenaar	Gassner et al., 2010 [74]	67	3
Vrouwenpolder	This study	96	41
Total		1527	177

Tick lysates were tested for the presence of Bartonella DNA using the gltA-primers. Samples were analysed by gel electrophoresis. PCR products of 380 bp were sequenced. Only Bartonella-related DNA sequences <99% similar to EF662054 were detected.

ISSN: 1756-3305