Hemolytic effect of def1 and def2. Hemolytic activity was carried out using 2% (vol/vol) erythrocyte suspension to evaluate all mild impacts on the erythrocyte membrane . Normally, the 4-5% (vol/vol) erythrocyte suspension is used for the hemolysis experiments [41, 42]. Significant differences between negative controls and peptides are marked by an asterisk (p < 0.05). No differences between hemolysis by def1 and def2 were statistically determined.