Figure 2
From: Functional characterization of two defensin isoforms of the hard tick Ixodes ricinus
Life cycle and tissue gene expression of I. ricinus def1 and def2 isoforms. Semi-quantitative two steps RT-PCR was performed with specific primers for each defensin. Tissues for examination were obtained from blood fed female and were as follows: HM-hemocytes, SG-salivary glands, MG - midguts, MT - Malphigian tubes, OV - ovaries. Negative control was done without cDNA. Life stages of I. ricinus tick used were larvae, nymphs and adult females (N-unfed, F-fed). Gene for tick β-actin was used as a positive control.