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Table 1 Minimal inhibition concentrations (MICs) and minimal microbicidal concentrations (MMCs) of def1 and def2 isoforms.

From: Functional characterization of two defensin isoforms of the hard tick Ixodes ricinus

Bacterial, yeast and viral species MIC [μM] MMC [μM]
  def1 def2 def1 def2
Bacillus subtilis (G+) 1.5 0.75 2 1
Micrococcus luteus (G+) 0.75 0.37 10 5
Staphylococcus luteus (G+) 50 25 50 25
Escherichia coli (G-) no effecta no effecta   
Pseudomonas aeruginosa (G-) no effectb no effectb   
Candida albicans (yeast) no effectb no effectb   
TBEV (Flaviviridae) no effectb no effectb no effectb no effectb
WNV (Flaviviridae) no effectb no effectb no effectb no effectb
  1. Determination of MICs and MMCs for each peptide and bacteria was performed at least 3-times in doublets. Def2 peptide was significantly more efficient in the inhibition of growing G-positive bacteria (G+) in cultures and in microbicidal experiments. Gram negative bacteria (G-), as well as yeast, WNV and two strains of TBEV (Hypr and Neudoerfl), were not influenced by these peptides (a no effect up to 50 μM; b no effect up to 100 μM).