| |
Oxygen uptake
|
Oxygen uptake after succinate
|
Oxygen uptake after duroquinol
|
Oxygen uptake after TMPD/ascorbate
|
|---|
| |
nmol O
2
/min/mg protein
|
nmol O
2
/min/mg protein
|
nmol O
2
/min/mg protein
|
nmol O
2
/min/mg protein
|
|
Control
|
13.88889
|
___
|
___
|
___
|
|
Prodigiosin (5 mM)
|
2.46667
|
2.46667
|
6.53333
|
44.66667
|
|
TTFA (5 mM)
|
2.23333
|
ND
|
ND
|
ND
|
|
KCN (5 mM)
|
6.76722
|
ND
|
ND
|
ND
|
- Oxygen consumption was evaluated in control and treated parasites. In order to determine which complexes of the electron transport chain were involved in the abnormal respiration, we added electron donors for each one of the complexes. The untreated parasites showed a higher oxygen uptake with respect to prodigiosin TTFA- and KCN-treated parasites. However, when electron donors for complex II and III were added the oxygen uptake was increased.
