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Table 1 Sensitivity of the used sample preparations compared to the gold standard for identifying T. brucei s.l. by PCR (n = 300)

From: The best practice for preparation of samples from FTA®cards for diagnosis of blood borne infections using African trypanosomes as a model system

Material

True positive

False positive

True negative

False negative

% NPV (95%CI)

% Sensitivity (95%CI)

Kappa value

Whole blood on FTA®cards (10 separate discs)

36

0

199

65

75.4% (70.2-80.6)

35.6% (26.3-45)

0.4

Whole blood on FTA®cards (elution)

57

0

199

44

81.9% (77.1-86.7)

56.4% (46.8-66.1)

0.6

DNA extract

69

0

199

32

86.1% (81.7-90.6)

68.3% (59.2-77.4)

0.7

Lysed blood on FTA®cards (elution)

74

0

199

27

88.1% (83.8-92.3)

73.3% (64.6-81.9)

0.8

Lysed blood on FTA®cards (10 separate discs)

74

0

199

27

88.1% (83.8-92.3)

73.3% (64.6-81.9)

0.8