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Table 1 Sensitivity of the used sample preparations compared to the gold standard for identifying T. brucei s.l. by PCR (n = 300)

From: The best practice for preparation of samples from FTA®cards for diagnosis of blood borne infections using African trypanosomes as a model system

Material True positive False positive True negative False negative % NPV (95%CI) % Sensitivity (95%CI) Kappa value
Whole blood on FTA®cards (10 separate discs) 36 0 199 65 75.4% (70.2-80.6) 35.6% (26.3-45) 0.4
Whole blood on FTA®cards (elution) 57 0 199 44 81.9% (77.1-86.7) 56.4% (46.8-66.1) 0.6
DNA extract 69 0 199 32 86.1% (81.7-90.6) 68.3% (59.2-77.4) 0.7
Lysed blood on FTA®cards (elution) 74 0 199 27 88.1% (83.8-92.3) 73.3% (64.6-81.9) 0.8
Lysed blood on FTA®cards (10 separate discs) 74 0 199 27 88.1% (83.8-92.3) 73.3% (64.6-81.9) 0.8