Larval migration in PERL chambers as an in vitro model for percutaneous infection stimulates feeding in the canine hookworm Ancylostoma caninum

Table 1 Overview of analysed A. caninu m larval populations

Abbreviation	Population	Treatment
iL3	infective larvae	none; collected from coproculture
pmL3 +CO₂	in presence of CO₂ percutaneously migrated larvae	migration within the PERL chamber system; incubation at 37°C in presence of 5% CO₂
pmL3 -CO₂	percutaneously migrated larvae	migration within the PERL chamber system; incubation at 37°C without additional CO₂
ssL3 +CO₂	serum-stimulated larvae	incubation at 37°C in presence of 10% serum and 5% CO₂
ssL3 -CO₂	serum-stimulated larvae without CO₂	incubation at 37°C in presence of 10% serum but without additional CO₂
ssL3nc +CO₂	negative control for serum-stimulated larvae	incubation at 37°C without serum but in presence of 5% CO₂
ssL3nc -CO₂	negative control	incubation at 37°C without serum and without additional CO₂

ISSN: 1756-3305