Figure 3

Rooted phylogenetic tree showing the relationship of the T. simiae identified in this study with reference sequences of T. simiae, T congoloense and T. brucei. The relationship was determined using the ITS1 of rRNA gene sequences by neighbor joining with 1,000 bootstrap. T. simiae samples identified in this study were depicted in bold letters. Trypanosomes sequences from GenBank were shown both by their accession numbers and parasites names). Scale bar used was nucleotide substitutions per position